Analysis of cell wall polymer fractions in the <i>GhGalT1</i> overexpression transgenic <i>Arabidopsis</i> plants.

<p>(<b>A</b>) Non-cellulosic monosaccharide composition of alcohol insoluble residue (AIR) of rosette leaves from <i>GhGalT1</i> transgenic lines, wild type and mutants. (<b>B</b>–<b>E</b>) Sequential extraction of the extracted alcohol-insoluble residue (AIR) and analysis of monosaccharide composition. AIR of rosette leaves from <i>GhGalT1</i> transgenic lines, wild type and mutants was sequentially extracted using CDTA (<b>B</b>), Na₂CO₃ (<b>C</b>), 1 M KOH (<b>D</b>), and 4 M KOH (<b>E</b>). Arabinose (Ara), rhamnose (Rha), xylose (Xyl), mannose (Man), galactose (Gal), glucose (Glc), galacturonic acid (GalA) and glucurnic acid (GlcA) are expressed as a percentage of the respective fractions. WT: wild type; GhGalT1oe-L1 and -L5: <i>GhGalT1</i> overexpression transgenic line 1 and 5; SALK 043252 and SALK 015338: two T-DNA insertion mutants.