Mechanical stretch decreases IL-10 receptor and IL-10 gene expression and increases SOCS3.

<p>E18 type II epithelial cells were exposed to 20% cyclic stretch for 24 hours. (<b>A</b>) Samples were analyzed by real-time PCR for IL-10 receptor mRNA expression (n = 5, *P<0.0001, **P<0.0001 vs their respective controls). (<b>B</b>) By Western blot for IL-10 R1 protein abundance (n = 4, *P<0.05 vs control). (<b>C</b>) By real-time PCR for IL-10 gene expression (n = 4, *P<0.05). (<b>D</b>) By real-time PCR for SOCS3 gene expression (n = 3, *P<0.05).