EdU uptake occurs in neurons and glia <i>ex vivo</i>.

<p>Flow cytometric analysis was performed on LMMP that was cultured with EdU for 72 h and gated for single cells that co-expressed tdT and the specified neuronal or glial marker (white histogram). EdU uptake was detected in 54% of Tuj/tdT⁺ cells (A), 64.7% of PGP9.5/tdT⁺ cells (B), 62.1% of S100ß/tdT⁺ cells (C), and 73.1% of GFAP/tdT⁺ cells. The gray histogram represents LMMP cultured with EdU for only 24 h was used as a negative control. After culturing LMMP for 48 h in media containing EdU then chasing for 5 days in differentiating media without EdU, immunofluorescence revealed cells with EdU uptake (green) that immunostained for neuronal and glial markers (red) including Tuj (E), PGP9.5 (F), S100ß (G), GFAP (H), nNOS (I), and VIP (J). Scale bars: 50 µm in E-J.