<p>(A) The salt bridge formed between RapJ residues Arg105 and Glu87, and the bonds between RapJ residues Glu126, Lys123, and Tyr161, in the RapJ-PhrC structure are depicted. RapJ is depicted in cartoon format, and the surface of PhrC is shown. In vivo activity of the RapJ (B) and RapH mutants (C) containing mutations targeting the Phr-dependent intramolecular Rap protein surfaces. Rap activity was measured as a function of P<i>spoIIG</i>::<i>luc</i> expression (see <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001512#pbio.1001512.s001" target="_blank">Figure S1A and S1B</a>). The inset panels show the Spo0A∼P activity of the P<i>spoIIG::luc</i> strains in the absence of induced RapJ or RapH. Each curve is repre...
<p>(A) Superimposition of RapF structures from the PhrF (blue hues) and ComA (orange hues) <a href="...
Two-component systems, composed of a sensor histidine kinase and an effector response regulator (RR)...
<p>(A) Side (left) and top view (right) of the superimposition of RapF 3-helix bundle from the PhrF ...
<p>(A) Schematic representation of the RapJ-PhrC interface. RapJ residues are depicted with green an...
<p>(A) RapJ activity was measured as a function of P<i>spoIIG</i>::<i>luc</i> expression in strain V...
<p>The structures depicted show a Rap protein alone (bottom left, RapI), Rap-target protein complexe...
<p>(A) Close view of the PhrF binding site that is presented in two halves dissected along the pepti...
<p>Rap proteins shown an exquisite specificity for their inhibitory peptides as is exemplified by th...
<p>(A) RapF or its mutational variants were incubated with increasing concentrations of PhrF and/or ...
<p>(A) The X-ray crystal structure of RapJ (rainbow cartoon with α-helices depicted as cylinders) co...
<div><p>The large family of Gram-positive quorum-sensing receptors known as the RNPP proteins consis...
<p>(A) Native gel assays. The interaction of native RapF and the RapF mutants Asn227Ala (RapF<sup>N2...
<p>The <i>spo0L892</i> mutant was isolated in the early 1970s (J.A. Hoch, unpublished data) (A) and ...
15 páginas, 7 figuras.Two-component systems, composed of a sensor histidine kinase and an effector r...
Two-component systems, composed of a sensor histidine kinase and an effector response regulator (RR)...
<p>(A) Superimposition of RapF structures from the PhrF (blue hues) and ComA (orange hues) <a href="...
Two-component systems, composed of a sensor histidine kinase and an effector response regulator (RR)...
<p>(A) Side (left) and top view (right) of the superimposition of RapF 3-helix bundle from the PhrF ...
<p>(A) Schematic representation of the RapJ-PhrC interface. RapJ residues are depicted with green an...
<p>(A) RapJ activity was measured as a function of P<i>spoIIG</i>::<i>luc</i> expression in strain V...
<p>The structures depicted show a Rap protein alone (bottom left, RapI), Rap-target protein complexe...
<p>(A) Close view of the PhrF binding site that is presented in two halves dissected along the pepti...
<p>Rap proteins shown an exquisite specificity for their inhibitory peptides as is exemplified by th...
<p>(A) RapF or its mutational variants were incubated with increasing concentrations of PhrF and/or ...
<p>(A) The X-ray crystal structure of RapJ (rainbow cartoon with α-helices depicted as cylinders) co...
<div><p>The large family of Gram-positive quorum-sensing receptors known as the RNPP proteins consis...
<p>(A) Native gel assays. The interaction of native RapF and the RapF mutants Asn227Ala (RapF<sup>N2...
<p>The <i>spo0L892</i> mutant was isolated in the early 1970s (J.A. Hoch, unpublished data) (A) and ...
15 páginas, 7 figuras.Two-component systems, composed of a sensor histidine kinase and an effector r...
Two-component systems, composed of a sensor histidine kinase and an effector response regulator (RR)...
<p>(A) Superimposition of RapF structures from the PhrF (blue hues) and ComA (orange hues) <a href="...
Two-component systems, composed of a sensor histidine kinase and an effector response regulator (RR)...
<p>(A) Side (left) and top view (right) of the superimposition of RapF 3-helix bundle from the PhrF ...