<i>Acinetobacter baumannii</i> strain M2 secreted Hcp.

<p>A. Growth curves of strain M2 and the isogenic <i>tssB</i> and <i>hcp</i> mutants. Three biological replicates were performed; error bars denote the standard error of the mean of the biological replicates. B. Concentrated culture supernatants from strain M2, M2Δ<i>tssB</i> and M2Δ<i>hcp</i> analyzed by SDS-PAGE and Coomassie staining. A band corresponding to the approximate molecular mass of Hcp (∼19 kDa), indicated by the arrow, was observed in the sample derived from strain M2 and was absent in the samples from M2Δ<i>tssB</i> and M2Δ<i>hcp</i>. Mass spectrometry confirmed that the major protein in this band was Hcp. C. Western blot analysis of whole cell lysates and concentrated culture supernatants of <i>A. baumannii</i> strains. Hcp, indicated by the arrow, was detected in whole cell lysates of strain M2, M2Δ<i>tssB</i> and the complemented <i>tssB</i> mutant as well as in concentrated culture supernatants of strain M2 and the complemented <i>tssB</i> mutant.