Confirmation of the screening system of the shRNA library by using the shRNA directed against human Ago2 (pcPURU6i-Ago2).

<p>(A) General schematic diagram of the screening procedure. (B) Schematic diagram of the optimization for the screening condition using Ago2 as a positive control. (C) The inhibitory effects of the shRNA directed against human Ago2 (pcPURU6i-Ago2) on RNAi. The second transfection was performed at 72 h, 80 h and 88 h after the first transfection, and the luciferase activity was determined at 80 h, 88 h and 96 h after the first transfection. Data was shown as mean and S.D. (n = 3). *<i>P</i><0.05.