Cardiac ATP contents and Atp5g2 expression levels were reduced in AMPKα2 KO mice during CR.

<p><b>A.</b> Cardiac ATP contents were determined with a colorimetric/fluorometric assay kit (Abcam, San Francisco, CA). Data are expressed as mean ± SE and analyzed by two-way ANOVA. <b>B.</b> Western blot analysis of proteins involved in regulating mitochondrial function and dynamics.