Role of RKIP in cervical cancer cells response to cisplatin.

<p><b>A)</b> Representative pictures of the nonlinear regression analysis of cervical cancer cell lines treated with cisplatin for determination of half maximal inhibitory concentrations (IC₅₀). <b>B)</b> Graphic representation of the mean IC₅₀ values for cisplatin in the cervical cancer cell lines. The transfected cells with shRKIP were less sensitive to cisplatin treatment in the three different cell lines. <b>C)</b> HeLa transfected cell lines were exposed to increasing concentrations of cisplatin by 24 hours. Cisplatin treatment induced ERK activation (p-ERK1/2) and apoptosis of the cells as evaluated by PARP (total and cleaded specific antibodies) and caspase-9 cleavage, mainly in the empty vector transfected cells. All the experiments were done in triplicate at least three times. Data in the panels A and B are represented as the mean ± SD.