miR-125b-5p facilitates tumorigenesis <i>in vivo</i>.

<p>(A) Linear regression analysis of the <i>in vivo</i> tumor volume in 12 NSG mice (n = 6/group) after xenotransplantation of MyLa cells transfected with miR-125b-5p or scrambled oligonucleutide. The tumor growth rate represented by the slope of the linear regression was statistically different in the miR-125b-5p group compared to the scrambled one (<i>P</i> = 0.03). Each point represents a single measurement. (B) Kaplan-Meier survival curve showing a significant (Log-Rank Mantel Cox test <i>P</i> = 0.04) shorter median survival in the miR-125b-5p group compared to the scrambled one. (C) miR-125b-5p expression in miR-125b-5p and scrambled tumors was quantified by qRT-PCR (*<i>P</i>< 0.05). Each point represents the miR-125b-5p level in one tumor tissue and the line corresponds to the median value of miR-125b-5p in the miR-125b-5p and scrambled tumors. (D) A representative ISH from 6 cases of paraffin sections of miR-125b-5p or scrambled tumors using miR-125b-5p-specific LNA probe. miR-125b-5p expression was high in all tumors within the miR-125b-5p group, while it was almost undetectable in the tumors within the control group. Images were acquired using a Nikon D60 digital single-lens reflex (D-SLR) camera. Original magnification x20 for both panels. Scale bar, 10 µm.