LRRC6 expression in ciliated airway epithelial cells.

<p>Photomicrographs of normal human lung section (<b>A</b>) (scale bar = 100 µm) and bronchial epithelium (<b>B</b>) following immunofluorescent staining for LRRC6, which reveals the cytoplasmic localization of the protein (LRRC6, red) only in ciliated cells (acetylated α-tubulin, a cilia marker, green; DAPI: blue) (scale bar = 10 µm). Immunofluorescent staining of nasal epithelial cells cultured at an air-liquid interface from a healthy subject demonstrating cytoplasmic localization of LRRC6 (<b>C</b>) as compared to decreased expression in a cell from a PCD subject (<b>D</b>). LRRC6 was similarly present in a non-targeted shRNA (NT) treated airway epithelial cells (<b>E</b>) but absent in <i>LRRC6-specific shRNA</i> transfected cells (<b>F</b>) (scale bar = 10 µm). Ultrastructural appearance of cilia from a normal control (<b>G</b>), PCD subject (<b>H</b>)<b>,</b> and airway epithelial cells following transfection with non-targeted (<b>I</b>) and <i>LRRC6-</i>targeted (<b>J</b>) shRNA sequences. Arrow and arrow-head indicate inner and outer dynein arms, respectively.