Persistence of unrecombined HGF mRNA and protein in the livers of HGF^{ex.5 flox}; Cre^+/− mice after genomic recombination.

<p>(<b>A</b>) Schematic of the targeting strategy for conditional inactivation of the gene for HGF (top). Cre-mediated excision of the floxed HGF allele (middle) leads to the generation of a recombined allele (bottom) lacking exon 5. (<b>B</b>) Successful genomic deletion of HGF exon 5 after induction of recombination, as shown by PCR. Top - HGF^{ex.5 flox};Cre-ER^T mice; bottom - HGF^{ex.5 flox};Mx1-cre mice. (<b>C</b>) RT-PCR shows the presence of both recombined and unrecombined HGF mRNA in KO livers. (<b>D</b>) WB for HGF in control and HGF KO livers shows no differences after recombination. Ponceau represents loading control.