Recombination occurs in all hepatic cell populations, including those that produce HGF.

<p>(<b>A</b>) Separation of hepatic cell populations from HGF^{ex.5 flox};Mx1-cre mice into hepatocytes and NPCs shows recombination in both after p(I):p(C) treatment as compared to controls. (<b>B</b>) RT-PCR shows persistence of unrecombined HGF mRNA in both hepatocytes and NPCs. (<b>C</b>) Real-time PCR for full-length HGF mRNA shows a decrease in HGF in the NPC fraction after p(I):p(C) treatment. (<b>D</b>) WB for HGF in hepatocytes and NPCs shows that the amount of HGF is unchanged in KOs compared to controls, and is found mainly in the NPC fraction. Ponceau represents loading control.