Effect of activin A on miR-181a expression and mouse granulosa cell (mGC) proliferation.

<p>mGC was isolated from 21-day-old mouse ovaries. (A) mGC was treated with indicated concentrations of activin A for 24 h. MiR-181a level was determined by qRT-PCR. (B) qRT-PCR analysis was performed to measure miR-181a level in mGC treated with activin A (50 ng/ml) for up to 48 h. (C) The proliferation of mGC was measured by CCK-8 after treated with activin A for 48 h. Cyclin D2 mRNA (D) and protein (E) levels were examined in mGC after treated with activin A (50 ng/ml) for 48 h by qRT-PCR and Western blotting, respectively. Relative protein levels were measured by densitometry using Quantity One Software and normalized to β-actin, the control group; the ratios were presented above the Western blot bands. All experiments were performed three times. *p<0.05, **p<0.01, compared with untreated controls.