Relationship between meconium FAEE concentration and ethanol-induced changes in fetal organs (endpoint).

<p><i>Note:</i> Pathology analyses were conducted in instrumented animals receiving ethanol and their appropriate instrumented controls that received saline (groups 1 and 2 listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059168#pone-0059168-t001" target="_blank">Table 1</a>). Organ pathology findings based on treatment group assignment (ethanol vs. control) have been previously reported in their respective publications, which are cited for each organ of interest. Herein, the animals are grouped according to their meconium FAEE concentrations, rather than treatment received. All animal pathology data is displayed as mean ± SEM, with an <i>n</i> value of 4–9 fetuses per group depending on the outcome in question. The mRNA data are presented as fold change relative to control. Lung mRNA data were log-transformed prior to statistical analysis. Spearman correlation analysis between meconium FAEE concentration and anatomical/physiological endpoints in individual animals was conducted using data from ethanol-exposed fetuses and saline controls, with an <i>n</i> of 9–15 fetuses depending on the endpoint in question. Abbreviations: Collagen I α1 (COLIα1), surfactant protein (SP), interleukin (IL), left ventricle plus septum (LV+S), tumour necrosis factor alpha (TNF-α).</p>a<p>For detailed methodology and results based on treatment received, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059168#pone.0059168-Gray1" target="_blank">[18]</a>.</p>b<p>For detailed methodology and results based on treatment received, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059168#pone.0059168-Sozo1" target="_blank">[19]</a>.</p>c<p>For detailed methodology and results based on treatment received, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059168#pone.0059168-Goh2" target="_blank">[17]</a>.</p>d<p>For detailed methodology and results based on treatment received, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059168#pone.0059168-Kenna1" target="_blank">[20]</a>.</p>*<p>Unpaired Student’s t-test unless otherwise specified.</p>‡<p>Mann-Whitney U test.</p>†<p>Spearman correlation.