Suppressive effect of LT on CFU-MK formation.

<p>(A) The experimental outline. Human cord blood-derived mononuclear cells (CBMCs) were used in a megakaryocytic colony-forming unit (CFU-MK) assay. This figure shows the morphology (B–C) and quantified numbers (D) of megakaryocytic colonies. Arrowheads in the low magnification images (B-1 to B-6) indicate specific colonies that are highlighted in high-magnification photographs (C-1 to C-6) for each group (C). Scale bar: B, 2 mm; C, 200 µm. The cell-culture medium was used to dilute LT and was used as a treatment control in vehicle-groups. Data are representative of 3 independent experiments. One plate was seeded with 1.1×10⁵ cells. Data are reported as mean ± standard deviation (SD), **<i>p</i><0.01 compared to vehicle groups.