DMXAA acts as a potent adjuvant.

<p>(A) Anti-OVA tIgG titers of C57BL/6 mice immunized with 100 µg OVA plus the indicated doses of DMXAA (µg). (B) Anti-OVA tIgG titers of C57BL/6 mice 21 days and 150 days after immunization with 100 µg OVA and 100 µg DMXAA. (C–E) C57BL/6 mice were immunized twice i.d. with 100 µg OVA plus DMXAA (100 µg), Alum (665 µg) or CpG DNA (25 µg) and the induction of (C) tIgG, (D) IgG1 and (E) IgG2c antibody responses against OVA were assessed. (F) IFN-γ secretion from splenocytes of immunized mice that were stimulated for 48 h with CD4 and CD8 OVA peptides and whole OVA protein. (G) Anti-OVA tIgG titers of C57BL/6 mice injected i.v. with 200 µg anti-CD4 (GK1.5) antibodies prior to immunization with 100 µg OVA and 100 µg DMXAA. Results presented are representatives of three separate experiments. *<0.05, ** <i>P</i><0.01, *** <i>P</i><0.001 by Students t-test when comparing between two groups and one-way ANOVA with Bonferroni's post-test when comparing three or more groups.