Adjuvant effect of DMXAA can improve potency of influenza SV vaccine and protect mice from a lethal challenge.

<p>C57BL/6 mice were immunized twice i.d. with 0.75 µg SV and 100 µg DMXAA, and their sera were assessed for anti-SV (A) tIgG, (B) IgG1 and (C) IgG2c antibodies. ** <i>P</i><0.01 by Students t-test. Results presented are pooled from two separate experiments. (D) C57BL/6 mice that had received two i.d. injections of PBS (open square), 100 µg DMXAA alone (open circle), 0.75 µg SV alone (grey-filled circle) or SV plus DMXAA (black filled circle) were challenged with a lethal dose of A/Puerto Rico/8/34 (PR) (H1N1) 7 days after the final immunization (n = 6 mice per experimental group). Results presented are pooled from two separate experiments. Survival rates were recorded daily and statistical analyses were performed using the log-rank (Mantel-Cox) test where ** and *** denotes p<0.01 and p<0.001 respectively (E) The rate of weight-loss by the challenged mice were monitored and presented as an average percentage of the initial base weight ± standard error. * denotes p<0.05 vs SV alone by Student's T-test.