FXR1P depletion in C2C12 cells and in myoblasts derived from FSHD myopathic patients biopsies contributes to a consistent increase in <i>p21</i> mRNA that translates into enhanced p21 protein levels.

<p>(A) Quantitative RT-PCR reveals a significant increase of <i>P21</i> mRNA in FSHD myoblats relative to control individuals. Data are presented as means ± SEM of n = 3 individuals/group. (B) Representative western-blot of p21 protein levels in siControl (siC) or siFxr1 (siFx)-transfected C2C12 cells. Densitometric quantification of western-blots reveal that depletion of FXR1P by siRNA transfection (siFxr1) leads to a significant increase of p21 protein levels relative to siControl-transfected cells. Data are presented as means ± SEM of n = 4 experiments. (C) Representative western-blot of P21 protein levels in FSHD patients and control individuals. Densitometric quantification of western-blots reveals that muscle biopsies of FSHD patients display a significant increase of P21 protein relative to controls. Data are presented as means ± SEM of n = 3 individuals/group. The asterisks * and ** indicate respectively p<0.05 and p<0.01 of the Mann & Whitney test.