CGJ up-regulates eNOS mRNA expression in coronary artery endothelial cells.

<p>Endothelial cells were incubated either with different concentrations of CGJ for 8 hours (A) or CGJ (11 mg/l) in a time-dependent manner until 8 hours (B). Then total mRNA was extracted and transcripted into cDNA. The level of eNOS mRNA was determined using RT-PCR analysis. n = 6 different experiments. CGJ did not affect eNOS mRNA stability (C). Actinomycin (15 µg/mL), an inhibitor of transcription, was added to endothelial cells in the presence or absence of CGJ (11 mg/l). n = 4 different experiments *<i>P</i><0.05 versus control.