Loss of DNA-PKcs impairs phosphorylation of IKKβ, IκBα, NF-κB and JNK in response to CpG-ODN.

<p>(<b>A</b>) WT, DNA-PKcs^−/− (PKcs^−/−), TLR9^−/− and PKcs^−/−/TLR9^−/− DCs were treated with CpG (5 µg/ml) for the indicated time durations or left untreated. Cell lysates were prepared and phospho-IKKα/β and beta-actin were detected by an immunoblotting (IB) analysis. (<b>B</b>) WT, DNA-PKcs^−/− (PKcs^−/−), TLR9^−/− and PKcs^−/−/TLR9^−/− DCs were treated with CpG (5 µg/ml) for the indicated time durations or left untreated. The phospho-IκBα, phospho-NF-κB and phospho-JNK as well as JNK and beta-actin were detected. Similar results were obtained from at least three independent experiments.