BRG1 knockdown reduces breast cancer cells proliferative ability.

<p>A Forty-eight hours after transfection, the expression of BRG1 in MDA-MB-231 and BT-549 cells was evaluated by Western blot. Cul1 was used as a nucleoprotein loading control. B, C CCK-8 cell proliferation assay was performed after BRG1 knockdown in MDA-MB-231 and BT-549. D, E The percentage of G1 population cells was measured by flow cytometry after BRG1 knockdown in MDA-MB-231 and BT-549 cells. F Western blot analysis of the relative protein levels of cyclin D1, cyclin E and p27 in BRG1 knockdown and control group of MDA-MB-231 and BT-549 cells. β-Actin was used as a whole cell protein loading control. All experiments were carried out in triplicate. Data are shown as mean ± SE. <b>**</b><i>P</i><0.01; <b>***</b><i>P</i><0.001.