Histone acetylation and HDAC binding at RGS10-1 promoters in chemoresistant A2780-AD cells and parental A2780 cells.

<p>ChIP assays were carried out in A2780 parental cells and multi-drug resistant A2780-AD. Lysates were immunoprecipitated with control, anti-acetyl histone H3, anti-acetyl H3K18, or anti-HDAC1 antibody. Associated DNA was isolated and analyzed via real time PCR using primers spanning the RGS10-1 and GAPDH promoters. Real-time PCR values were normalized to the total amount of promoter DNA added (input). Input values represent 5% of the total cell lysate. * P<0.05. <b>A.</b> Global levels of Histone H3 acetylation associated with RGS10 and GAPDH promoters in A2780 and A2780-AD ovarian cancer cells. Values represent mean ± SEM of four independent experiments. <b>B.</b> Levels of histone H3 acetylated at lysine 18 associated with RGS10-1 and GAPDH promoters in A2780 and A2780-AD ovarian cancer cells. Values represent mean ± SEM of four independent experiments. <b>C.</b> Levels of HDAC1 associated with RGS10 and GAPDH promoters in A2780 and A2780-AD ovarian cancer cells. Values represent mean ± SEM of three independent experiments. <b>D.</b> Western blot analysis of global HDAC1 levels in A2780 and A2780-AD cells.