Expression and function of miR-541 and miR-155 during hBMSC/MSC osteogenesis.

<p>(A) Expression pattern of miR-541 and miR-155 with (osteo) or w/o (control) osteo-induction. (B) Knock-down of miR-541 and miR-155. miRNA levels were normalized to Snord48 levels. (C) Effect of anti-miR-541 or anti-miR-155 on <i>OPN/SPP1</i> and <i>ALP</i> mRNA expression. Values were normalized to S29 mRNA levels. (D) Effect of anti-miR-541 and anti-miR155 on calcification (top row) and alkaline phosphatase production (ALP, bottom 2 rows). The thick stained part in the center by alizarin red S was re-organized in the 2nd row.