YC-1 Inhibits the Expression of <i>NFκB/p65, SDF-1, CXCR4, αV, α5,</i> and <i>β1</i> in VEGF Stimulated Cells. A–F. Real Time RT-PCR Analysis.

<p>The mRNA levels for <i>NFκB/p65, SDF-1, CXCR4, FAK, αV, α5, β3, β1, EPO, ET-1, MMP-9,</i> was quantified by Real time RT-PCR. The mRNA levels of these genes were upregulated in the cells that were cultured in the presence of VEGF [30 ng/ml] and either treated with DMSO [0.2%], or SN50M [20 µM]. Whereas the cells that were cultured in medium only exhibited significant low mRNA levels. Treatment of VEGF-stimulated cells with SN50 [20 µM] or YC-1 [100 µM] resulted in a significant downregulation of the mRNA expression of the genes listed above, as compared to respective controls; SN50M and DMSO-treated cells, respectively. ANOVA was used for statistical analyses. Mean ± SEM of mRNA level normalized to β-actin were calculated, [***<i>P</i><0.001 and **<i>P</i><0.01, as compared to respective controls]. Data are representative of 3 independent experiments.