YC-1 Inhibits the Expression of Various Pro-Angiogenic Molecules in VEGF-Stimulated Cells.

<p><b>A–E. Real Time RT-PCR Analysis.</b> The mRNA levels for <i>β3, FAK, EPO, ET-1, and MMP-9,</i> was quantified by Real time RT-PCR. The expression level was upregulated in the cells that were cultured in the presence of VEGF and either treated with DMSO or SN50M. Treatment with SN50 or YC-1 resulted in a significant downregulation of the mRNA expression, as compared to their respective controls; SN50M and DMSO-treated cells, respectively. ANOVA was used for statistical analyses. Mean ± SEM of mRNA level normalized to β-actin were calculated, [***<i>P</i><0.001 and **<i>P</i><0.01, as compared to respective controls]. Data are representative of 3 independent experiments.