Bulbous arteriosus defects in <i>tbx1^−/−</i> embryos.

<p>Dissected hearts from 72 hpf embryos showing the BA region (A, D) outlined in white, with the bidirectional arrowheads in black and red showing the length and width of BA, respectively. (B, E) The corresponding images showing staining for Alcama antibody (red) and DAF-2DA (green). (C, F) <i>tbx1^−/−</i> mutants at the same stage have absent <i>eln2</i> expression. Black arrows indicate the BA. (G) Quantification of BA width in WT and <i>tbx1−/−</i> mutants, while (H) demonstrates that BA length is reduced in <i>tbx1^−/−</i> mutants; * p-value = 0.0045. N = 13 for all measurements. Scale bars: 25 µm.