Substitutions detected in four chemostats over 50 days.

<p>S43a and S43b were grown on <i>S. typhimurium</i> as host at 43.5°; E37a and E37b were grown on <i>E. coli</i> at 37°. Order by row is based upon the number of chemostats in which the substitution arose, then on the earliest 10 day sample in which it was detected and then by position in the genome. The column labeled ‘180’ indicates the presence of a substitution in E37a after 180 days of evolution <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060401#pone.0060401-Wichman3" target="_blank">[27]</a>. The last four columns indicate the number of other chemostat experiments (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060401#pone.0060401-Wichman1" target="_blank">[17]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060401#pone.0060401-Bull3" target="_blank">[24]</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060401#pone.0060401-Wichman2" target="_blank">[26]</a>, unpublished data) in which the substitution arose at each environmental condition (S = <i>S. typhimurium</i>, E = <i>E. coli</i>, 42≥42°, 37 = 37°). Red indicates missense substitutions, blue silent substitutions and green substitutions in intergenic regions. ^aPreviously presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060401#pone.0060401-Wichman3" target="_blank">[27]</a>.