Macrophage depletion in mice impaired type 2 immunity induced by exogenous IL-33.

<p>A–C, IL-33-induced upregulation of IL-13 and IL-5 was attenuated in the small intestine (A), spleen (B), and mesenteric lymph nodes (C), in mice treated with Cl₂MDP. D, E, IL-33-induced smooth muscle hypercontractility was attenuated in mice with decreased number of macrophage as shown by the response to electric field stimulation (D, EFS) and the amplitude of spontaneous contraction (E, SC) (n = 5 per group, *p<0.05 vs respective BSA; ^φp<0.05 vs PBS-IL-33, one-way ANOVA followed by Neuman-Keuls test; p = 0.002 was generated from two-way ANOVA with repeated measures between the two curves). Data shown in bar and line graphs are the means ± s.e.m, and are representative of two independent experiments.