TGF-beta signaling inhibits VEGFA expression and suppresses tumor angiogenesis <i>in vivo</i>.

<p>Representative images of IHC staining of phospho-Smad2 (A), VEGFA (B) and CD31 (C) in the primary tumors of FETα/vector and FETα/DNRII cells (left panels). Staining density was measured and quantified with ImagePro plus 7.0 Software using 20× or 40× images (right panels). Four animals were analyzed for each type of cells. Fifteen to twenty histologically similar fields were randomly selected from each slide for analysis of staining density. The data are presented as the mean ± SE. <i>P</i> values were calculated using Student's <i>t</i>-test. * <i>P</i><0.001.