Expression of VEGFA inversely correlates with TGF-beta signaling in human colon cancer specimens.

<p>Sections prepared from 10 normal colon and 24 individual colon cancer patient specimens were stained using anti-phospho-Smad2 and anti-VEGFA antibodies. (A) Representative images of IHC staining of phospho-Smad2 and VEGFA in normal colon and colon cancer specimens. (B) Staining density was measured and quantified with ImagePro plus 7.0 Software using 40× images in each sample. Normal colon samples are represented by squares and colon cancer samples by triangles. Bars represent mean values of each group of samples. <i>P</i> values were calculated using Student's <i>t</i>-test. * <i>P</i><0.001.