Assay scheme.
- Silvia Galbiati (394159)
- Francesco Damin (394160)
- Pamela Pinzani (116725)
- Irene Mancini (394161)
- Serena Vinci (394162)
- Marcella Chiari (394163)
- Claudio Orlando (116744)
- Laura Cremonesi (394164)
- Maurizio Ferrari (394165)
Publication date
March 2013
DOIAbstract
<p>Hybridization steps: 1) stabilizer oligonucleotide to open the secondary structure of the PCR fragment; 2) The “universal reporter mix”. The mix contains the wild-type and mutant reporters. Each reporter is prolonged by a tail complementary to the labeled universal oligonucleotide. Cyanine 3- (Cy3) and Cyanine 5- (Cy5) labeled universal oligonucleotide anneal to wild-type and mutant reporters, respectively.</p
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