<p><b>A</b>, <i>Left</i>, Schematic shows placement of split YFP on GIRK2c and GIRK3. Note the C-terminal domains are free to interact with other proteins. <i>Right</i>, BiFC-tagged GIRK2c/3 channels are functional. Current-voltage plot is shown for <sup>CY</sup>GIRK2c/<sup>NY</sup>GIRK3 channels. Baclofen (100 µM) activates and Ba<sup>2+</sup> (1 mM) inhibits inwardly rectifying current. HEK293T cells were transfected with cDNA encoding GABA<sub>B1a</sub>, GABA<sub>B2</sub> and <sup>CY</sup>GIRK2c/<sup>NY</sup>GIRK3. Average baclofen-induced current densities were –13.2±6.0 pA⋅pF<sup>−1</sup> (n = 3) for <sup>CY</sup>GIRK2c/<sup>NY</sup>GIRK3. <b>B</b>, HEK293 cells were co-transfected with <sup>CY</sup>GIRK2c, <sup>NY</sup>GIRK3 and eithe...
<p>In resting state (no activated GPCR), the GIRK1/2 channel, a heterotetramer of 2 GIRK1 (grey) and...
<p><b>(A)</b> Cellular localization of YFP-tagged wild-type canine CNGA3 and CNGA3-V644del mutant in...
AbstractFunctionally critical domains in the βγ-subunits of the G-protein (Gβγ) do not undergo large...
<p><b>A</b>, Cartoon shows model of GIRK channels regulation by SNX27b. GIRK channels recycle throug...
<p><b>A</b>, Alignment of two different RA domains: a RID (Ras interacting domain) from RalGDS and a...
<p><b>A</b>, Schematic shows GIRK2c and GIRK3 with PDZ-binding motif and GIRK2a and GIRK3-RR, which ...
G protein-gated inwardly rectifying potassium (GIRK) channels play an important role in regulating n...
SummaryThe subcellular pathways that regulate G protein-gated inwardly rectifying potassium (GIRK or...
G protein‐activated K⁺ channel (GIRK) subunits possess a conserved extracellular integrin‐binding mo...
(A) IHKE-1 cells were cultured on glass cover slips for 24 hours, fixed and subsequently stained wit...
<p>(A) In situ hybridization showing regions of β4 expression in a mouse brain. Arrow points to high...
AbstractG protein-coupled inwardly rectifying potassium channels (GIRK/Kir3) are important elements ...
(A) Amino acid sequences of i3 of mutant and chimeric constructs of MC9-YFP (black: M1R sequences, r...
AbstractThe G protein-coupled inward rectifier K+ channel (GIRK) is activated by direct interaction ...
(A) Representative whole-cell patch clamping recording traces in monoclonal HEK293T cells stably exp...
<p>In resting state (no activated GPCR), the GIRK1/2 channel, a heterotetramer of 2 GIRK1 (grey) and...
<p><b>(A)</b> Cellular localization of YFP-tagged wild-type canine CNGA3 and CNGA3-V644del mutant in...
AbstractFunctionally critical domains in the βγ-subunits of the G-protein (Gβγ) do not undergo large...
<p><b>A</b>, Cartoon shows model of GIRK channels regulation by SNX27b. GIRK channels recycle throug...
<p><b>A</b>, Alignment of two different RA domains: a RID (Ras interacting domain) from RalGDS and a...
<p><b>A</b>, Schematic shows GIRK2c and GIRK3 with PDZ-binding motif and GIRK2a and GIRK3-RR, which ...
G protein-gated inwardly rectifying potassium (GIRK) channels play an important role in regulating n...
SummaryThe subcellular pathways that regulate G protein-gated inwardly rectifying potassium (GIRK or...
G protein‐activated K⁺ channel (GIRK) subunits possess a conserved extracellular integrin‐binding mo...
(A) IHKE-1 cells were cultured on glass cover slips for 24 hours, fixed and subsequently stained wit...
<p>(A) In situ hybridization showing regions of β4 expression in a mouse brain. Arrow points to high...
AbstractG protein-coupled inwardly rectifying potassium channels (GIRK/Kir3) are important elements ...
(A) Amino acid sequences of i3 of mutant and chimeric constructs of MC9-YFP (black: M1R sequences, r...
AbstractThe G protein-coupled inward rectifier K+ channel (GIRK) is activated by direct interaction ...
(A) Representative whole-cell patch clamping recording traces in monoclonal HEK293T cells stably exp...
<p>In resting state (no activated GPCR), the GIRK1/2 channel, a heterotetramer of 2 GIRK1 (grey) and...
<p><b>(A)</b> Cellular localization of YFP-tagged wild-type canine CNGA3 and CNGA3-V644del mutant in...
AbstractFunctionally critical domains in the βγ-subunits of the G-protein (Gβγ) do not undergo large...