A comparison of antibody responses of mice vaccinated with DNA expressing L1 or L1+L2 of HPV6, 16, or 18, either singly or together at the same or different sites.

<p>Balb/c mice were vaccinated i.m. with electroporation three times at two week intervals with 20 µg each of DNA expressing L1 or L1+L2 of HPV6, HPV16, and HPV18, either individually, or together at the same site or each at a different site (DS), or s.c. with Gardasil. Serum samples were harvested at two weeks after the third vaccination (A–C) or 3 months after the third vaccination (D–F), and neutralizing antibody titers were measured with HPV6 (A,D), HPV16 (B,E), or HPV18 PsV (C,F). Antibody response to L2 was measured by ELISA against full length HPV16 L2 with serum samples collected two weeks after the third vaccination (G).