HCMV upregulates p53 and p21 in HepG2 cells and PHH.

<p>Time course of p53, p21 and Mdm2 in HepG2 cells and PHH infected with HCMV. HepG2 cells (6×10⁶ cells) and PHH (2×10⁶ cells) were left uninfected or infected with HCMV strains AD169 and HCMV-DB at MOI = 0.5 and 1, respectively. P53, p21 and Mdm2 protein expressions were measured by Western blotting, and beta-actin was used as an internal control. Results are representative of two independent experiments.