Id1 knockdown in MDCK-EGFP-E47 cells induces a partial MET and impairs cell survival.

<p>(<b>A</b>) Western blot analysis of Id1 expression in MDCK-EGFP-E47 cells after 48 h infection with shId1 or control lentivirus (EV), compared to non-infected (EGFP-E47) and control MDCK-CMV (CMV) cells; alpha-tubulin was used as loading control. (<b>B</b>) Phase contrast (upper panels) and confocal immunofluorescence images of E-cadherin (lower panels) of EGFP-E47-shId1 (right) and EGFP-E47-EV control (middle) cells 48 h after lentiviral infection; non infected MDCK-EGFP-E47 cells (left) are shown as additional control. Nuclei were stained with DAPI; bars, 50 um. (<b>C</b>) Cell cycle analysis of the indicated cell lines by propidium iodide staining and FACS analysis. (<b>D</b>) Apoptosis rate of the indicated cells determined by the sub-G0 population. Results in <b>C</b> and <b>D</b> represent the mean +/− s.d. on triplicate samples.