Rfg1, Brg1, and Efg1 regulate white-opaque switching in <i>MTL</i>a/α strains.

<p>(A) PCR of <i>MTL</i><b>a</b>1 and α2 genes of the <i>rfg1/rfg1</i>, <i>brg1/brg1</i>, and <i>efg1/efg1</i> mutants. Primers used for PCR are listed in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001525#pbio.1001525.s010" target="_blank">Table S6</a>. Three characterized WT strains served as <i>MTL</i><b>a</b>/α (SN250), α/α (WO-1), and <b>a</b>/<b>a</b> (GH1012) controls. (B) White-to-opaque switching in <i>rfg1/rfg1</i>, <i>brg1/brg1</i>, and <i>efg1/efg1</i> mutants (<i>MTL</i><b>a</b>/α) on Lee's GlcNAc plates. WT (SN250, <i>MTL</i><b>a</b>/α), a derivative of SC5314, served as a control. The three mutants were also with the same background of SC5314. Switching frequencies of white-to-opaque are shown below the colony images.