<p>A) All mutp53fl proteins selectively recognized scBSK by EMSA. P53 proteins (protein amount expressed as p53/total DNA ratio) were incubated with scBSK (200 ng, lanes 1, 3–25) and linBSK (200 ng, lanes 2–25) and separated on a 1.3% 0.33x TBE agarose gel at 4°C (linDNA migrated faster than scDNA). P53-DNA binding was detected by Et-Br staining of DNA. The conformation of p53 proteins is labeled: white (wtp53), grey (conformation mutants) and black (contact mutants). B) Binding of CΔ30-wtp53 (lanes 4–7), CΔ30-G245S (lanes 8–11), CΔ30-R248W (lanes 12–15) and CΔ30-R175H (lanes 16–19) to pBSK in sc/lin competition asssay was performed similarly to p53fl (A). CΔ30-R248W and CΔ30-R175H lose the ability for strong SCS-binding. Column graph below...
<p><b>A</b>. EMSA assay testing double-stranded oligonucleotide probes containing the putative p53 b...
Despite the loss of sequence-specific DNA binding, mutant p53 (mutp53) proteins can induce or repres...
<p><b>Copyright information:</b></p><p>Taken from "Mutant p53 proteins bind DNA in a DNA structure-s...
<p>A) Mutp53 proteins recognize MSP/MST1 site in scDNA differently. Mutp53s and wtp53 were bound to ...
<p>A) Preferential binding of G245S to scMSP in sc/lin competition assay in H1299 cells by ChIP. pGL...
<p>A) Scheme of the procedure (from left to right): the cell lysate or purified proteins were mixed ...
<p><b>(A)</b> Scheme of intramolecular T.A.<i>T</i> triplex in scDNA. AFM image of sc pA69 plasmid a...
<p>A) Distribution of hot spot mutations on full length p53 (conserved regions marked by cylinders) ...
We showed previously that bacterially expressed full-length human wild-type p53b(1-393) binds select...
We showed previously that bacterially expressed full-length human wild-type p53b(1-393) binds select...
We showed previously that bacterially expressed full-length human wild-type p53b(1-393) binds select...
Hot spot mutant p53 (mutp53) proteins exert oncogenic gain-of-function activities. Binding of mutp53...
Hot spot mutant p53 (mutp53) proteins exert oncogenic gain-of-function activities. Binding of mutp53...
<div><p>Hot spot mutant p53 (mutp53) proteins exert oncogenic gain-of-function activities. Binding o...
<p>(<b>A</b>) Detection of p53-biding ability of TLP. TLP and TBP were examined for p53 binding by a...
<p><b>A</b>. EMSA assay testing double-stranded oligonucleotide probes containing the putative p53 b...
Despite the loss of sequence-specific DNA binding, mutant p53 (mutp53) proteins can induce or repres...
<p><b>Copyright information:</b></p><p>Taken from "Mutant p53 proteins bind DNA in a DNA structure-s...
<p>A) Mutp53 proteins recognize MSP/MST1 site in scDNA differently. Mutp53s and wtp53 were bound to ...
<p>A) Preferential binding of G245S to scMSP in sc/lin competition assay in H1299 cells by ChIP. pGL...
<p>A) Scheme of the procedure (from left to right): the cell lysate or purified proteins were mixed ...
<p><b>(A)</b> Scheme of intramolecular T.A.<i>T</i> triplex in scDNA. AFM image of sc pA69 plasmid a...
<p>A) Distribution of hot spot mutations on full length p53 (conserved regions marked by cylinders) ...
We showed previously that bacterially expressed full-length human wild-type p53b(1-393) binds select...
We showed previously that bacterially expressed full-length human wild-type p53b(1-393) binds select...
We showed previously that bacterially expressed full-length human wild-type p53b(1-393) binds select...
Hot spot mutant p53 (mutp53) proteins exert oncogenic gain-of-function activities. Binding of mutp53...
Hot spot mutant p53 (mutp53) proteins exert oncogenic gain-of-function activities. Binding of mutp53...
<div><p>Hot spot mutant p53 (mutp53) proteins exert oncogenic gain-of-function activities. Binding o...
<p>(<b>A</b>) Detection of p53-biding ability of TLP. TLP and TBP were examined for p53 binding by a...
<p><b>A</b>. EMSA assay testing double-stranded oligonucleotide probes containing the putative p53 b...
Despite the loss of sequence-specific DNA binding, mutant p53 (mutp53) proteins can induce or repres...
<p><b>Copyright information:</b></p><p>Taken from "Mutant p53 proteins bind DNA in a DNA structure-s...