Electroporation efficiency of different buffers in primary human T lymphocytes.

<p>(<b>A</b>) PBMCs from two healthy donors were electroporated using in house buffers and 4 µg of pT2-GFP plasmid. Cell viability and GFP expression were analyzed after 24h by flow cytometry. Cell viability is normalized to control (not electroporated) cells. Electroporation scores were determined as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060298#s2" target="_blank">materials and methods</a>. Values are the average of two donors in triplicate and are expressed as mean±SEM. Statistical analysis was performed using One Way ANOVA and Tukey post test (* = P<0.05). (<b>B</b>) Representative dot plots of GFP (pT2-GFP; 4 µg) expression 24 h after electroporation with 1SM buffer. Numbers in plots represents the percentage of cells in the gate. Gray line = negative control; black line = cells electroporated with pT2-GFP. (<b>C</b>) Electroplated lymphocytes were stained for CD4 and CD8 24 h after electroporation with 1SM buffer and 4 µg pT2-GFP plasmid. Data are representative of two donors. Numbers in plots represents the percentage of cells in the gate.