Knockdown of IRF7 enhances PR8 virus production in MDCK cells.

<p>(A) MDCK cells were transfected with siRNAs targeting the indicated genes. At 48 h post-transfection, the cells were infected with PR8 virus at a MOI of 0.01. After 24 h of incubation, viral RNA in culture supernatant was measured by quantitative real-time RT-PCR. The gray boxes in the panel mean that siRNA which targets indicated gene showed more than 2-fold enhancement of the virus production compared with negative control siRNA. (B) Total RNA from cells was extracted to monitor knockdown efficiency of target gene in the cells. The endogenous expression of target genes was measured by quantitative real-time RT-PCR with SYBR green. (C) MDCK cells were transfected with three siRNAs targeting genes or negative control siRNA. At 48 h post-transfection, the cells were infected with PR8 virus at a MOI of 0.01. After 24 h of incubation, the RNA from culture supernatant was isolated to examine the amounts of a specific viral RNA. The data are representative results of three independent experiments. Asterisks indicate statistically significant differences compared with the control (*P<0.05).