<i>Arabidopsis</i> UAP56 has ATPase acitivity that is stimulated by RNA and dsDNA.

<p><b>A</b> ATP hydrolysis by UAP56 (or the control protein HMGB2, each 8 µM) was tested in the presence (absence) of ssRNA (R13, 50 µM). ATP and ADP were separated by TLC and analysed by phosphorimaging. <b>B</b> Stimulation of the ATPase activity of UAP56 (8 µM) by different amounts of ssRNA was quantified from three independently repeated experiments, and error bars indicate standard diviations. <b>C</b> ATPase activity of different concentrations of recombinant UAP56 in the presence of ssRNA (R13, 50 µM). <b>D</b> Stimulation of the ATPase activity by different RNAs (cf. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060644#pone.0060644.s007" target="_blank">Table S2</a>). The effect of ssRNA vs. dsRNA and of short (13-nt) vs. longer (25-nt) RNA (50 µM each) on the ATPase activity of UAP56 (4 µM). As evaluated using Students t-test, the different RNAs stimulate the ATPase activity to different extents (P<0.05).<b>E</b> ATP hydrolysis of UAP56 in the absence of nucleic acids and in the presence of 13-nt ssRNA or 25-bp dsDNA.