Results of amplification used to estimate the yield of the <i>Hbb-b1</i> promoter–HS4/5 ligation product.

<p>(A) Primers used to amplify the <i>Hbb-b1</i> promoter–HS4/5 ligation junction. (B) Primers to an inner portion of the HS4/5 restriction fragment (control amplification). Fluorescence growth curves (logarithmic scale) are presented on the left graphs. Green curves show the results for the 3C template; blue curves show the results for the negative control (50 ng of mouse genomic DNA (A) or mQ (B)); red curves of different color intensity show the results for the DNA standards. Standard curves are presented on the right graphs. (C) The equation used to calculate the yield of ligation products. N_total–the quantity of each of the two restriction fragments; N_ligated–the quantity of the ligation product of these two fragments.