Methylation and expression of RASSF3 in rat and mouse normal pituitary samples and somatotroph adenoma cell lines.

<p>Methylation levels were investigated using pyrosequencing and are shown as an average level of the 10 CpG sites in the RASSF3 promoter region. RASSF3 mRNA expression fold was determined using qRT-PCR, and β-actin mRNA level was also analyzed as endogenous control. In both GH3 and GT1.1 cells, RASSF3 methylation level was higher and mRNA level was lower than in normal pituitary samples.