Effects of curcumin on TGF-β1-induced ERK phosphorylation in HK-2 cells.

<p>(A) HK-2 cells were stimulates with TGF-β1 (2.5 ng/ml ) for the indicated times, and the phosphorylation of ERK was analyzed by western blotting. (B) Western blotting analyses for p-ERK expression in HK-2 cells. HK-2 cells were incubated with curcumin at the indicated doses for 12 hours followed by treatment with TGF-β1 for 30 minutes. (C) Graphical presentation of the relative expression of pERK. The values were represented as the density of p-ERK vs total ERK (%). The mean ± SD (n = 3) of values were obtained from densitometric analysis of all individual experiments. ^#P<0.05 vs. control alone, *P<0.05 vs TGF-β1 alone.