Multivalent vectors elicit an <i>in vivo</i> anti-KWAS immune response.

<p>BALB/c mice (n = 8) were primed, boosted, and reboosted with 1×10¹⁰ VP of Ad vectors (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060347#pone-0060347-g004" target="_blank">Figure 4A</a>). A–B) Post-prime and post-reboost serum was collected at various time points for ELISA binding assays. 1 µM of purified KWAS (PCEWDEAELDKWASNLEEEDDDNE) antigenic peptide was bound to ELISA plates. Residual unbound peptide was washed from the plates. The plates were then incubated with immunized mice serum and the binding was detected with HRP conjugated secondary antibody. OD absorbance at 450 nm represents KWAS antibody levels in sera. The values are expressed as the mean ± standard deviation. The *** indicate a <i>P</i> value ≤ 0.001, and ** indicate a <i>P</i> value ≤ 0.01.