Schematic illustration of the experimental procedure.

<p>The transposons were mixed with the plasmids encoding the <i>Sleeping Beauty</i> (SB) transposase and were then hydrodynamically delivered to the liver (see the Materials and Methods section). Once in cells, the SB transposase is expressed and binds to the IR/DRs of the transposons. The enzyme subsequently cleaves the transposons at the sites of IR/DRs and integrates them at a new location within the host genome, allowing the transgenes to be stably expressed. Mice are then subjected to repeated bioluminescence imaging over time.