Effects of RNF-113 depletion on <i>C. elegans</i> survival after interstrand DNA crosslinking (ICL) and the intracellular localization of RNF-113.

<p>(A) Comparison of embryonic hatching rates after knocking down RNF-113 expression with and without ICL treatment. <i>rnf-113</i> RNA<i>i</i> was performed from the P0 young adult stage by feeding wild-type <i>C. elegans</i> worms <i>E. coli</i> cells expressing double-strand RNA for <i>rnf-113</i>. F1 worms at the L4 stage were treated with TMP (25 µg/ml) plus UVA, and eggs were collected over 24 h and their hatching scored 20 h later. This set of experiments in the wild-type background, was also performed in <i>fcd-2(tm298)</i> and <i>rfs-1(ok1372)</i> mutants. The error bars are SEM (standard errors of the mean). (B) Intracellular localization of RNF-113 in the germ cells of the mitotically proliferating region of <i>C. elegans</i> gonads was detected using polyclonal antibodies against RNF-113. Immuno-staining was performed at 3, 9, 16, and 24 h after ICL treatment in the wild type. The scale bar is 10 µm.