Sequence information of <i>Zic2</i> mutant alleles.

<p>A) Sequences obtained from the mutant blastocysts (CD1) or from the founder lines of mutant mice (C3H and C57BL/6J) generated following microinjection of TALEN-A mRNAs. B) Sequences obtained from the mutant blastocysts (CD1) or from the founder line of mutant mice (C57BL/6J) generated following microinjection of TALEN-B mRNAs. The DNA sequences to which the TALEN monomers were designed are highlighted in red. Nucleotide mutations and insertions (shown with arrows) are shown in lower case and are highlighted in green. The reading frame of the ZIC2 protein is shown above the wild-type sequences and the predicted consequences of the mutation on the amino acid sequences are shown to the right of the DNA sequences. Divergence (missense/deletion etc) from the wild-type peptide sequence is shown in green and premature Stop codons are shown as x. Critical cysteine and histidine residues of the 5^th zinc finger domain are underlined.