Plasmid maintenance in <i>E. coli</i> using the <i>glmS</i> system.
- Kwangsoo Kim (395907)
- Jae Ho Jeong (395908)
- Daejin Lim (395909)
- Yeongjin Hong (395910)
- Misun Yun (395911)
- Jung-Joon Min (395912)
- Sahng-June Kwak (395913)
- Hyon E. Choy (299112)
Publication date
March 2013
DOIAbstract
<p>GlmS<sup>−</sup> mutant bacteria (CKS1001) or the parental strain (CH1436) carrying <i><sup>E.c</sup>GlmS<sup>+</sup>p</i> were subcultured (1/1000) in minimal media every 12 hrs. The fraction of bacteria carrying <i><sup>E.c</sup>GlmS<sup>+</sup>p</i> at the indicated time was determined on GlcNAc-supplemented LB plates containing ampicillin (50 mg/ml).</p
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