Mutations that inhibit sumoylation of Rep1 and Rep2 also impair plasmid maintenance.

<p>Yeast lacking the native 2 µm plasmid were transformed with <i>KanMX6</i>-tagged 2 µm plasmids encoding the indicated Rep1 and Rep2 alleles, grown in medium containing G418 and the proportion of plasmid-containing (G418-resistant) cells (mean ± SEM from six independent yeast transformants) was determined.