Measurement of in vitro prophage induction.

<p>SIVET strains were grown in LB broth and serially diluted into fresh LB. Cultures were grown to desired densities and concentrations of CamR and KanR bacteria in cultures measured at indicated points in growth cycle. Multiple rounds of dilutions and growth allowed for indicated rounds of duplication. Where appropriate, low dilutions ensured that any CamR bacteria would be carried over during dilutions. Graphs show ratios of CamR/KanR on a log scale (calculated using the Induction Index formula) over the indicated number of generations. A. SIVET <i>cIind1</i>strains K11607 (locked in KanR form) and K11608 (CamR) were grown together in LB to stationary phase at which time bacterial counts were determined and dilutions were made for next round of growth. Error bars represent standard error of the mean. B. Cultures of K11604 were grown to stationary phase at which time bacterial counts were determined and dilutions were made for next round of growth. C. Cultures of K11604 were grown to late log phase at which time dilutions were made for next round of growth. Aliquots were removed at mid-log phase for determination of bacterial counts.